Coding

Part:BBa_M36108:Design

Designed by: Andee Wallace, Taylor Nguyen, Chad Viergever   Group: Stanford BIOE44 - S11   (2011-05-03)

Protein Generator, no promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 459
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 459
    Illegal NotI site found at 217
    Illegal NotI site found at 289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 459
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 459
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Composite part of a strong 5' UTR, the HY5 protein sequence, and a terminator sequence. This part does not include a promoter, which allows the anyone to use any promoter type (constitutive or regulatory) with it. The part solely expresses the HY5 protein.

Source

5' UTR

HY5 gene

Terminator sequence

References

Sources for HY5 protein:

HY5 amino acid sequence: http://www.uniprot.org/uniprot/O24646

Most prevalent e. coli codons: http://openwetware.org/wiki/Escherichia_coli/Codon_usage

Translating one letter amino code to actual amino acid names: http://www.mun.ca/biochem/courses/3107/aasymbols.html